Three-dimensional analysis of plant structure using high-resolution X-ray computed tomography.

نویسندگان

  • Wolfgang H Stuppy
  • Jessica A Maisano
  • Matthew W Colbert
  • Paula J Rudall
  • Timothy B Rowe
چکیده

High-resolution X-ray computed tomography (HRCT) is a non-invasive approach to 3D visualization and quantification of biological structure. The data, based on differential X-ray attenuation, are analogous to those otherwise obtainable only by serial sectioning. Requiring no fixing, sectioning or staining, HRCT produces a 3D digital map of the specimen that allows measurements and visualizations, including arbitrarily oriented sections. In spite of its application throughout the natural sciences, HRCT has yet to be applied in extant plant structural research.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assessment of X-Ray Crosstalk in a Computed Tomography Scanner with Small Detector Elements Using Monte Carlo Method

Introduction: Crosstalk is a leakage of X-ray or light produced in a matrix of X-ray detectors or array of photodiodes in one element to other elements affecting on image contrast and spatial resolution. In this study, we assessed X-ray crosstalk in a computed tomography (CT) scanner with small detector elements to estimate the effect of various parameters such as X-ray tube voltage, detector e...

متن کامل

Three-dimensional reconstruction of New Zealand rabbit antebrachium by multidetector computed tomography

The aim of this study was to reveal biometric peculiarities of New Zealand white rabbit antebrachium (radius and ulna) by means of three-dimensional (3D) reconstruction of multidetector computed tomography (MDCT) images. Under general anesthesia, the antebrachiums of a total of sixteen rabbits of both sexes were scanned with a general diagnostic MDCT. Biometric measurements of the reconstructed...

متن کامل

Three-dimensional visualization of a human chromosome using coherent X-ray diffraction.

Coherent x-ray diffraction microscopy is a lensless phase-contrast imaging technique with high image contrast. Although electron tomography allows intensive study of the three-dimensional structure of cellular organelles, it has inherent difficulty with thick objects. X rays have the unique benefit of allowing noninvasive analysis of thicker objects and high spatial resolution. We observed an u...

متن کامل

Using high resolution computed tomography to visualize the three dimensional structure and function of plant vasculature.

High resolution x-ray computed tomography (HRCT) is a non-destructive diagnostic imaging technique with sub-micron resolution capability that is now being used to evaluate the structure and function of plant xylem network in three dimensions (3D) (e.g. Brodersen et al. 2010; 2011; 2012a,b). HRCT imaging is based on the same principles as medical CT systems, but a high intensity synchrotron x-ra...

متن کامل

Design of Small Animal Computed Tomography Imaging for in vitro and in vivo Studies

Introduction: Mini Computed Tomography (mini-CT) was suggested in biomedical research to investigate tissues and small animals. We present designed and built a mini x-ray computed tomography (mini-CT) for small animals as well as industrial component imaging. Materials and Methods: The system used in this study includes a X-ray tube 20kV to 160kV and a flat pa...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Trends in plant science

دوره 8 1  شماره 

صفحات  -

تاریخ انتشار 2003